TLS Proxy has command line interface, however it writes tunnel definitions into the config.yaml file.
You can either delete the config.yaml file when you want clean proxy config:
del .\config\config.yaml
Or you can run tlsproxy tunnel delete -i 0 to delete the first tunnel. So, you can do something like this:
tlsproxy tunnel delete -i 0
tlsproxy tunnel add ...
tlsproxy run